Bromley Ladies who were crowned Division 2 (South) Champions in season 2013/14 have enjoyed three seasons in Division 1 with the 2015/16 campaign being their most successful spell.
The Kent side will be looking to make it third time lucky in their bid for a move the Step 4.
The 2015/16 Surrey Division Winners joined the GLWFL last season, finishing the season in sixth place after a very promising start.
The young side will use their experiences this season and will prove to be tough opposition.
Hampton & Richmond Borough
Hampton & Richmond are heading into their third season in Division one, who will also be looking to make it third time lucky.
The local side who are only down the road, will meet this season for the first time since Ashford's existence in 2014.
Luton Town Development
The Hatters finished last season just above Bromley and are another experienced Division 1 side who will be looking to improve on last seasons final league position.
The Bees were just pipped to promotion last season, after finishing in fourth place behind Hackney, Tottanham Development and Victorie Ladies. No doubt, Brentford will be looking to go one better this year with the arrival of their new manager.
The experience within the senior side should get them over the line this time and no doubt it'll be a tasty affair when Ashford and Brentford meet this season.
The last time Ashford played Clapham United was two seasons ago in the semi final of the Capital Cup. The Tangerines were the only team that season to beat Clapham.
The South-West London side enjoyed a successful campaign boasting a 100% league record, winning every game.
New London Lionesses
The Lionesses won Division 3 (North) in 2015/16 as well as Division 2 (North) in 2016/17.
They boast a similar record as The Tangerines and will be goaling for another league title this campaign as they head into their first season in Division 1.
Le Club Des Sports
Previously known as Battersea & Hammersmith, Le Club Des Sports have achieved back to back promotions and will be hoping for a hatrick this season.
The South London side played Ashford in the most recent GLWFL Trophy Final leading 2-0 at half time. The Tangerines however produced the come back of the season, winning the game 3-2.
Le Club Des Sports will be a strong side this season.
Arguably the strongest side in the Division this season, having retained their players and added strong additions early on.
Godalming enjoyed a good season in Division 2 (South) with Ashford and always proved to be tough competition when the two sides met.
The G's were close to securing a first win against Ashford, before Sequoia Belworthy struck late on.
Updated 08:19 - 23 Apr 2018 by William Boye